Method and apparatus for microscopy imaging with resolution correction

A method for microscopic imaging, comprising:

• providing a sample;
• illuminating the sample with illumination radiation for producing detection radiation;
• capturing the detection radiation to acquire at least one first image;
• computing a second image from the at least one first image using a correction algorithm;
• wherein the at least one first image has a first resolution, and the second image has a second resolution higher than the first resolution, wherein the acquiring at least one acquired first image comprises assembling a set of original images, wherein the set of original images are generated by using structured light illumination with different illumination directions having different phase information;
• the set of original images including one or more original images that are generated by the detection radiation caused by the illumination radiation with different illumination directions having different phase information, wherein the
• acquiring at least one first image further comprises the following steps:
• based on said assembling the set of original images, reconstruct the original image using a first reconstruction algorithm to obtain the first image, wherein the first reconstruction algorithm is a three-phase reconstruction algorithm and/or a four-phase reconstruction algorithm, the at least one first image having data of intensity information of the detected radiation of the sample, and
• in the same detection area of the sample, the at least one first image is acquired at a specified time interval, wherein the at least one first image is configured as an image sequence sorted according to the specified time interval.