Digital dispense system cartridge

A method for digitally dispensing fluid onto a fluid droplet substrate, comprising:

• providing a fluid droplet ejection system housed in a housing unit, the fluid droplet ejection system including:
• an open fluid droplet ejection cartridge having a cartridge body with one or more fluid chambers disposed therein, each of the one or more fluid chambers containing a fluid to be dispensed by the fluid droplet ejection system,
• a fluid cartridge translation mechanism for moving the fluid droplet ejection cartridge back and forth over a fluid droplet substrate in an x direction,
• a substrate translation mechanism for moving the fluid droplet substrate back and forth beneath the fluid ejection cartridge in a y direction orthogonal to the x direction, and
• an activation switch on the housing unit in electrical communication with a power input,
• wherein the open fluid droplet ejection cartridge comprises a cover attached to the cartridge body, the cover having one or more openings therein, fluid funnels pend from each of the one or more openings in the cover into the cartridge body, a chamber separator is attached to the fluid funnels for directing fluid into the one or more fluid chambers, a fluid overflow area is disposed between the one or more fluid chambers and the cartridge body for fluid overflow from the one or more fluid chambers, a fluid supply slot is provided in fluid flow communication with each of the one or more fluid chambers, and a plurality of fluid ejection devices is disposed on a semiconductor substrate attached to an outside portion of the cartridge body opposite the cover, wherein the plurality of fluid ejection devices are in fluid flow communication through the semiconductor substrate with the fluid supply slots;
• pipetting one or more fluids into the one or more fluid chambers of the open fluid droplet ejection cartridge;
• activating the fluid droplet ejection system using the activation switch;
• and
• digitally dispensing one or more fluids onto the fluid droplet substrate.