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US 11,768,198 B2Granted
Compositions and methods for molecular labeling
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren R. Link
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Claims11
A method for protein detection, the method comprising:
- introducing a protein-specific binding reagent linked to an oligonucleotide barcode to a sample comprising a population of cells;
- encapsulating the cells in sample droplets such that each sample droplet comprises a single cell and said protein-specific binding reagent by merging droplets containing said cells and said protein-specific binding reagents;
- and
- determining the presence of proteins of said cells that are bound to said protein-specific binding reagents.
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Dependencies are inferred from the claim text.