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US 11,768,156 B2Granted
Methods and compositions for assessing cell viability
AssigneeLife Technologies Corporation
InventorsKyle Gee, Kathleen Kihn, Yi-Zhen Hu, Yexin Wu, Kathleen Free
Life of this patent
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Abstract
Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.
Claims20
A compound of Formula I:
- D-L-R (I)
- wherein:
- D is a fluorescent moiety;
- L is a linker;
- and
- R is Formula IB:
- -A-Pt(X)(R3)(R4) (Formula IB),
- wherein A is a heterocycloalkyl or substituted heterocycloalkyl in which a nitrogen is bonded to the platinum of Formula IB;
- the substituents of the platinum of Formula IB are in a square planar configuration with R3 and R4 in cis;
- X is Cl, Br, or I;
- R3 and R4 are each independently —NH2R6, wherein R6 is H, C1-6 alkyl, or substituted C1-6 alkyl;
- or R3 and R4, together with the platinum of Formula IB, form a ring in which the two atoms bonded to the platinum are nitrogen and the ring comprises at least two carbons between the nitrogens.
9 more elements
Dependencies are inferred from the claim text.