Size-based separation of dissociated fixed tissues
AssigneeVentana Medical Systems, Inc.
InventorsNelson Alexander, Aoune Barhoumi, Lisa Gallegos, Stacey Stanislaw
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Abstract
The present disclosure provides a method of separating cellular particles from a tissue sample and then sorting the cellular particles into two or more cellular particle populations.
Claims21
A method of segregating cellular particles from a representative sample comprising:
- (i) obtaining the representative sample, wherein the representative sample is derived from at least one fixed tissue specimen that has been homogenized, wherein any heterogeneity of cells within the at least one fixed tissue specimen is substantially uniformly distributed within the representative sample;
- and (ii) sorting cellular particles in at least a first aliquot of the representative sample by size into at least a first cellular particle population and a second cellular particle population, wherein the cellular particles are not tagged or labeled prior to or during the step of sorting.
A method of segregating cells from a homogenized sample comprising:
- (i) obtaining a homogenized sample, wherein the homogenized sample is derived from one or more fixed tissue specimens, and where any aliquot of the homogenized sample substantially uniformly expresses the heterogeneity of the one or more fixed tissue specimens;
- and
- (ii) sorting untagged or unlabeled cells in the homogenized tissue sample by size, wherein the cells are sorted into at least a first cellular population enriched in cells having an average diameter of less than 12 μm and a second cellular population enriched in cells having an average diameter of greater than 12 μm, and wherein the cells within the homogenized sample are sorted using a microfluidic device.
A method of segregating cellular particles from a sample comprising sorting cellular particles in the tissue sample by size into at least a first cellular particle population and a second cellular particle population, wherein the cellular particles are not tagged or labeled prior to or during the step of sorting;
- wherein the sample is derived from at least one fixed tissue specimen, and where any aliquot of the sample substantially homogeneously expresses the heterogeneity of the at least one fixed tissue specimen.
Dependencies are inferred from the claim text.