Compositions and methods for detection of Babesia
AssigneeRoche Molecular Systems, Inc.
InventorsEllen H. Fiss, Jingtao Sun
Life of this patent
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Abstract
Methods for the rapid detection of the presence or absence of Babesia in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting Babesia and kits are provided that are designed for the detection of Babesia, including, but not limited to, the Babesia species of B. microti, B. divergens, B. duncani, and B. venatorum. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of Babesia.
Claims5
A method of detecting the Babesia species of (i) B. microti;
- and/or (ii) B. divergens, B. duncani, and/or B. venatorum in a sample, the method comprising:
- (a) performing an amplification step comprising contacting the sample with one or more set of primers to produce an amplification product, if a target nucleic acid of (i) B. microti, and/or (ii) B. divergens, B. duncani, and/or B. venatorum is present in the sample;
- (b) performing a hybridization step comprising contacting the amplification product, if the target nucleic acid of (i) B. microti, and/or (ii) B. divergens, B. duncani, and/or B. venatorum is present in the sample, with one or more probes;
- and
- (c) detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of (i) B. microti, and/or (ii) B. divergens, B. duncani, and/or B. venatorum in the sample, and wherein the absence of the amplification product is indicative of the absence of (i) B. microti, and/or (ii) B. divergens, B. duncani, and/or B. venatorum in the sample, and
- wherein the one or more set of primers and the one or more probes comprise:
- (1) a set of primers for amplification of B. microti comprising:
- a primer comprising the nucleic acid sequence of SEQ ID NO:1, or a complement thereof, and
- a primer comprising the nucleic acid sequence of SEQ ID NO:3, or a complement thereof, and
- a probe for hybridizing to the amplification product of B. microti comprising the nucleic acid sequence of SEQ ID NO:2, or a complement thereof;
- and/or
- (2) a set of primers for amplification of B. divergens, B. duncani, and/or B. venatorum comprising:
- a primer comprising the nucleic acid sequence of SEQ ID NO:4 or a complement thereof, and
- one or more primers compromising the nucleic acid sequence(s) of SEQ ID NO:6 SEQ ID NO:7 or a combination of SEQ ID NOs:6 and 7, or a complement thereof;
- and
- probe for hybridizing to the amplification product of B. divergens, B. duncani, and/or B. venatorum comprising the nucleic acid sequence of SEQ ID NO:5, or a complement thereof.
Dependencies are inferred from the claim text.