Use of detection reagent for detecting methylation of genes associated with colorectal cancer, and kit

A kit for auxiliary diagnosis of colorectal cancer, comprising a detection reagent for detecting methylation of a colorectal cancer-related gene;

• the colorectal cancer-related gene is one selected from the group consisting of SDC2 gene, TFPI2 gene and a combination thereof;
• the detection reagent contains a first reagent for detecting methylation of a first target region on the SDC2 gene or/and a second reagent for detecting methylation of a second target region on the TFPI2 gene,
• wherein the first target region is a region c,
• wherein the region c is selected from a full-length region and a partial region of chr8:96494006-96494335;
• and
• the second target region is a region d,
• wherein the region d is selected from a full-length region and a partial region of chr7:93890026-93890319,
• wherein the first reagent contains a first nucleic acid group for detecting methylation of the first target region, and the first nucleic acid group contains a combination of a fluorescent probe c and a primer pair c or a combination of a fluorescent probe c1 and a primer pair c1;
• the second reagent contains a second nucleic acid group for detecting methylation of the second target region, and the second nucleic acid group contains a combination of a primer pair d and a fluorescent probe d or a combination of a primer pair d1 and a fluorescent probe d1;
• wherein base sequences of the primer pair c consist of nucleotide sequences of SEQ ID NOs:
• 11-12, and a base sequence of the fluorescent probe c consists of a nucleotide sequence of SEQ ID NO:
• 13;
• and base sequences of the primer pair c1 consist of nucleotide sequences of SEQ ID NOs:
• 14-15, and a base sequence of the fluorescent probe c1 consists of a nucleotide sequence of SEQ ID NO:
• 16;
• and
• base sequences of the primer pair d consist of nucleotide sequences of SEQ ID NOs:
• 17-18, and a base sequence of the fluorescent probe d consists of a nucleotide sequence of SEQ ID NO:
• 19;
• and base sequences of the primer pair d1 consist of nucleotide sequences of SEQ ID NOs:
• 20-21, and a base sequence of the fluorescent probe d1 consists of a nucleotide sequence of SEQ ID NO:
• 22.