Microorganism and method for producing triacylglycerol
AssigneesHiroshima University, Tokyo Institute of Technology, Mazda Motor Corporation
InventorsAtsushi Sakamoto, Kumiko Okazaki, Takashi Yamamoto, Hiroyuki Ohta, Koichi Hori, Shinsuke Shimizu, Akihide Takami, Seiji Nomura, Fumihiko Saito
Life of this patent
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Abstract
A microorganism having at least one SPX gene encoding SPX protein responsive to phosphorus deficiency, and characterized in that a function of the SPX protein is decreased or lost by introducing gene mutation into the SPX gene.
Claims6
An alga belonging to the genus Nannochloropsis having at least one SPX gene encoding an SPX protein responsive to phosphorus deficiency, characterized in that a function of the SPX protein is decreased or lost by introducing a gene mutation into the SPX gene, the SPX gene encodes SEQ ID NO:
- 2, and the gene mutation deletes at least one amino acid residue selected from K250 (lysine at position 250 in SEQ ID NO:
- 2), R313 (arginine at position 313 in SEQ ID NO:
- 2), R315 (arginine at position 315 in SEQ ID NO:
- 2), K330 (lysine at position 330 in SEQ ID NO:
- 2), E466 (glutamic acid at position 466 in SEQ ID NO:
- 2), and K498 (lysine at position 498 in SEQ ID NO:
- 2).
An alga belonging to the genus Nannochloropsis having at least one SPX gene encoding an SPX protein responsive to phosphorus deficiency, characterized in that a function of the SPX protein is decreased or lost by introducing a gene mutation into the SPX gene, the SPX gene encodes an amino acid sequence having an SPX domain and 60% or more homology with SEQ ID NO:
- 2, and the gene mutation deletes at least one amino acid residue corresponding to one selected from K250 (lysine at position 250 in SEQ ID NO:
- 2), R313 (arginine at position 313 in SEQ ID NO:
- 2), R315 (arginine at position 315 in SEQ ID NO:
- 2), K330 (lysine at position 330 in SEQ ID NO:
- 2), E466 (glutamic acid at position 466 in SEQ ID NO:
- 2), and K498 (lysine at position 498 in SEQ ID NO:
- 2).
Dependencies are inferred from the claim text.