Adeno-associated virus complex with improved expression of RUNX3 gene and uses for preventing or treating KRAS mutated lung cancer
AssigneeGenecraft Inc.
InventorsSuk Chul Bae, You Soub Lee, Xinzi Chi, Ja Yeol Lee
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Abstract
Provided is an adeno-associated virus (AAV) complex for expression of an RUNX3 gene including an asymmetrically modified inverted terminal repeat (ITR). The AAV complex has asymmetric ITRs in which one of the two ITRs is modified, thereby increasing self-replication efficiency in host cells and increasing expression efficiency of a delivered gene, and therefore, compared to existing AAV complexes, the AAV complex has an advantage of improved productivity and gene expression efficiency.
Claims14
An adeno-associated virus (AAV) complex, comprising a polynucleotide sequence encoding a runt-related transcription factor 3 (RUNX3) protein between a first inverted terminal repeat (ITR) and a second ITR,
- wherein the first ITR is not modified and the second ITR is modified, and
- wherein the second ITR, all or part of a stem-loop structure, which is formed of rep-binding element (RBE), RBE′, A, A′, B, B′, C, C′, and D regions, is modified.
Dependencies are inferred from the claim text.