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US 11,767,528 B2Granted
Targeted trans-splicing using CRISPR/Cas13
AssigneeMassachusetts Institute of Technology
InventorJacob Borrajo
Life of this patent
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Abstract
This disclosure provides compositions and methods of using these compositions to mediate a targeted trans-splicing event on a pre-mRNA in a cell.
Claims19
A system for targeting a nucleic acid for trans-splicing, the system comprising:
- (i) a CRISPR/Cas system comprising a Type VI Cas polypeptide and a nucleic acid guide;
- (ii) a specific RNA-binding polypeptide that associates with the CRISPR/Cas system;
- and
- (iii) a trans-splicing template comprising a splice donor and/or acceptor and an RNA sequence that binds to the specific RNA-binding polypeptide, thereby targeting the nucleic acid for trans-splicing.
1 more element
Dependencies are inferred from the claim text.