US 11,767,523 B2Granted

Acrylamide copolymerization for sequestration and production of single-stranded nucleic acid

AssigneeDana-Farber Cancer Institute, Inc.

InventorsElisha Krieg, William M. Shih

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Provided herein, in some embodiments, are methods, compositions and kits for large-scale production of long single-stranded DNA in solution.

Dependent claims

A method of producing single-stranded deoxyribonucleic acid (ssDNA), comprising:

(a) combining in solution acrylamide-labeled double-stranded deoxyribonucleic acid (dsDNA) and acrylamide (AA) monomers to form copolymer-linked dsDNA in solution;
(b) dehybridizing the copolymer-linked dsDNA in solution to produce free ssDNA and DNA-containing copolymer;
and
(c) separating the free ssDNA from the solution of (b).

1 more element

Dependencies are inferred from the claim text.

C12N 15/1006
Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses…
C12N 15/111
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: general methods applicable to biologically…
C08F 220/56
Amides , e.g. N,N-dimethylacrylamide or N-isopropylacrylamide: acrylamide; Methacrylamide
C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…

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