Genetically modified bacterial cells and methods useful for producing indigoidine
AssigneeThe Regents of the University of California
InventorsThomas T. Eng, Deepanwita Banerjee, Aindrila Mukhopadhyay
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Abstract
The present invention provides for a genetically modified bacterial host cell capable of producing indigoidine, wherein the host cell comprises a non-ribosomal peptide synthetase (NRPS) that converts glutamine to indigoidine, and the bacterial host cell is reduced in its expression of one or more of the sixteen indicated enzymes.
Claims18
A genetically modified Pseudomonas host cell capable of producing indigoidine, wherein the host cell comprises a Bacillus subtilis 4′-phosphopantetheinyl transferase (Sfp) and a non-ribosomal peptide synthetase (NRPS) comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:1, and the amino acid sequence SRRLEREV (SEQ ID NO:3), or SRRLEREVAQESSRLVRLHAE (SEQ ID NO:4), at the position corresponding to 1005 to 1012, or 1005 to 1025, of SEQ ID NO:1, respectively, wherein the NRPS converts glutamine to indigoidine, and the Pseudomonas host cell is reduced in its expression of the following enzymes:
- Glucose dehydrogenase (ubiquinone 8 as acceptor, periplasm);
- Phosphoenolpyruvate synthase;
- Malate dehydrogenase;
- Malate dehydrogenase (ubiquinone 8 as acceptor);
- Malic enzyme NADP (malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+));
- and Transaldolase.
Dependencies are inferred from the claim text.