Engineered amylase variants
AssigneesCodexis, Inc., Societe des Produits Nestle S.A.
InventorsChinping Chng, Nikki Dellas, Da Duan, Ravi David Garcia, Harvinder Chagger Maniar
Life of this patent
Estimating the term…
Abstract
The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.
Claims18
A recombinant amylase comprising a polypeptide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 1728, wherein the polypeptide sequence comprises at least a substitution 272G/K/P/R/S, 394E/G/N/R/S, or 91H, or any combinations thereof, wherein the amino acid positions are relative to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.