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US 11,767,516 B2Granted
Methods and compositions for thiol-acrylate based matertals for 3D cell culturing in a microfluidic device
AssigneeBoard of Supervisors of Louisiana State University
InventorsAdam Melvin, John Pojman, Anowar Khan
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Abstract
Provided are thiol-acrylate hydrogels and tunable cell culture materials including thiol-acrylate hydrogels, and methods of making thereof. Also provided are systems for forming three-dimensional cell culture scaffolds including the materials, and methods of culturing cells, including cancer cells, using thiol-acrylate hydrogels and tunable cell culture materials. The materials herein can be used in microfluidic droplet-generating devices.
Claims18
A tunable cell culture material comprising a hydrogel,
- wherein the hydrogel is a product of a reaction between a thiol and an acrylate;
- wherein the thiol comprises ethoxylated trimethylolpropane tri (3-mercapto-propionate) (ETTMP), and the acrylate comprises poly(ethylene glycol) diacrylate (PEGDA);
- and
- when the hydrogel weight percent is about 8.5 and the molar ratio is about 1.0, the gelation time is about 150 minutes;
- when the hydrogel weight percent is about 8.5 and the molar ratio is about 1.05, the gelation time is about 40 minutes;
- when the hydrogel weight percent is about 9 and the molar ratio is about 1.0, the gelation time is about 80 minutes;
- when the hydrogel weight percent is about 9 and the molar ratio is about 1.05, the gelation time is about 30 minutes;
- when the hydrogel weight percent is about 9.5 and the molar ratio is about 1.0, the gelation time is about 30 minutes;
- and
- when the hydrogel weight percent is about 9.5 and the molar ratio is about 1.05, the gelation time is about 25 minutes.
7 more elements
A tunable cell culture material comprising:
- a hydrogel and a liquid medium;
- wherein the hydrogel is a product of a reaction between a thiol and an acrylate;
- wherein the thiol comprises ethoxylated trimethylolpropane tri (3-mercapto-propionate) (ETTMP), and the acrylate comprises poly(ethylene glycol) diacrylate (PEGDA);
- and
- wherein the liquid medium comprises an extracellular buffer (ECB) comprising the following components:
- HEPES, NaCl, KCl, MgCl2·6H2O, CaCl2·2H2O, and glucose.
3 more elements
Dependencies are inferred from the claim text.