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US 11,767,511 B2Granted
Platelets as delivery agents
AssigneeCellphire, Inc.
InventorsKeith Andrew Moskowitz, Amber Nicole Lee, Rafael Jorda
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Abstract
Provided herein are RNA agent-loaded platelets, methods of preparing RNA agent-loaded platelets, and methods of using RNA agent-loaded platelets. In some embodiments, methods of loading RNA agents into platelets include treating platelets with a RNA agent, a cationic transfection reagent, and a loading buffer that can include a salt, a base, a loading agent, and optionally at least one organic solvent.
Claims22
A method of preparing RNA agent-loaded freeze-dried platelet derivatives, comprising:
- (a) treating platelets with a RNA agent that is a siRNA and/or a miRNA, in the presence of a cationic transfection reagent, and a loading buffer comprising a salt, a base, and a loading agent comprising a monosaccharide and/or a disaccharide for a time period in the range of 20 minutes to 12 hours, and at a temperature in the range of 18-42° C., to form RNA agent-loaded platelets;
- and
- (b) lyophilizing the RNA agent-loaded platelets to form the RNA agent-loaded freeze-dried platelet derivatives, wherein the RNA agent-loaded freeze-dried platelet derivatives upon rehydration retain at least 10% of the RNA agent.
Dependencies are inferred from the claim text.