System and method for generating and testing tissue

A packaged bioreactor device comprising:

• a cup including a bottom, a top, and an internal cavity extending between the bottom and the top;
• a lid sealing the top of the cup and being removable to access the cavity;
• a tissue scaffold supported in the cavity;
• and
• a mask removably disposed in the cavity above the tissue scaffold, the mask including a plurality of through holes to access the tissue scaffold and a portion of the cavity below the mask;
• wherein the tissue scaffold is supported by a cassette removably disposed in the cavity of the cup below the mask;
• wherein the cassette includes a fixed clip engaging a first end of the tissue scaffold, and a second floating clip engaging a second end of the tissue scaffold, the floating clip detachably coupled to the cassette;
• wherein the mask includes a pair of downwardly extending first anti-rotation protrusions arranged to engage a mating pair of upwardly extending second anti-rotation protrusions formed on a bottom wall of the cup within the cavity, the mating anti-rotation protrusions preventing relative rotation between the mask, cassette, and cup.