Elisa for diagnosis of Haemonchus longistipes infection in camels
AssigneeKing Faisal University
InventorsSabry Mohamed Bahy El-Bahr, El Awad M. El Hassan, Mohammad H. Al-Omran
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Abstract
An ELISA for the diagnosis of Haemonchus longistipes infection in camels is provided. The ELISA for the diagnosis of Haemonchus longistipes infection in camels includes antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes. The resulting antibodies may be used in assays to detect Haemonchus longistipes infected camels. The assays may be any kind of enzyme-linked immunosorbent assay, or “ELISA”, known to those of skill in the art. The assay using the antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes infected camels may be capable of detecting pre-patent Haemonchus longistipes infection.
Claims7
A method of producing a Haemonchus longistipes (H. longistipes) specific antibody, comprising the steps of:
- (1) preparing H. longistipes crude extract;
- (2) isolating an approximately 76 kDa protein from the H. longistipes crude extract;
- (3) purifying the approximately 76 kDa protein by Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-page) and electro-elution;
- (4) raising antibodies against the approximately 76 kDa protein in a non-camelid mammal;
- and
- (5) purifying antibodies from the non-camelid mammal, wherein
- the step of raising antibodies against the approximately 76 kDa protein further comprises immunization and boosting steps, and
- the immunization step comprises injecting the non-camelid mammal with 100 μg of the 76 kDa protein on day 0 and day 14;
- and wherein the boosting step comprises injecting the non-camelid mammal with 50 μg of the 76 kDa protein on day 49 and 77, and a final injection of 25 μg of the 76 kDa protein on day 196.
Dependencies are inferred from the claim text.