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US 11,767,351 B2Granted
Recombinant lectin and uses thereof
AssigneeCity of Hope
InventorsMarkus Kalkum, Yoko Fujita-Yamaguchi, Karine Bagramyan, Teresa B. Hong, Sarah Shuck
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Abstract
Disclosed herein are a recombinant Streptomyces S27S5 hemagglutinin (SHA), and homologues thereof, and a fusion protein of a fluorescent protein (such as GFP and mCherry1) and SHA or a homologue thereof, which specifically bind to carbohydrates, including oligomeric sugars that terminate in L-rhamnose or D-galactose. The SHA, SHA homologues, and fusion proteins can be used to detect a variety of microorganisms or cancer or tumor antigens.
Claims6
A method of detecting a cancer or tumor in a subject, comprising:
- contacting a sample obtained from the subject with a fusion protein comprising a fluorescent protein and a Streptomyces hemagglutinin (SHA) protein or a homologue of the SHA protein, wherein the SHA protein has an amino acid sequence represented by SEQ ID NO:
- 25, the homologue of the SHA protein has an amino acid sequence at least 90%, at least 95%, at least 98%, or at least 99% identical to that of SEQ ID NO:
- 25, wherein the homologue of the SHA protein comprises SEQ ID NO:
- 17, SEQ ID NO:
- 19, and SEQ ID NO:
- 21;
- and wherein the fusion protein specifically binds to L-rhamnose or D-galactose;
- and
- detecting the fluorescence level in the sample, wherein the presence of fluorescence indicating the presence of the cancer or tumor,
- wherein the cancer or tumor cell expresses an antigen comprising a carbohydrate capable of specifically binding to the SHA protein, the homologue of the SHA protein, or an SHA protein fragment or a homologue of the SHA protein fragment.
7 more elements
Dependencies are inferred from the claim text.