Modified streptolysin O
AssigneeToyobo Co., Ltd.
InventorsYosuke Sumida, Hiroaki Kitazawa, Takahide Kishimoto
Life of this patent
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Abstract
The invention provides streptolysin O containing a polypeptide comprising an amino acid sequence having deletion of a polypeptide extending from a lysine residue at position 2 of SEQ ID NO: 1 to any of an alanine residue at position 31, a glutamate residue at position 32, a serine residue at position 33, and an asparagine residue at position 34, and also having deletion of any of an isoleucine residue at position 465 to an alanine residue at position 472 of SEQ ID NO: 1 and all of the subsequent amino acid residues, as well as variants thereof and related DNA, vectors, transformants, and methods of use thereof.
Claims8
A Streptolysin O consisting of a polypeptide having one or more tags in the N-terminal or C-terminal region of an amino acid sequence selected from position 32 to position 464 of SEQ ID NO:
- 1, position 32 to position 466 of SEQ ID NO:
- 1, position 32 to position 471 of SEQ ID NO:
- 1, position 35 to position 464 of SEQ ID NO:
- 1, position 35 to position 466 of SEQ ID NO:
- 1, and position 35 to position 471 of SEQ ID NO:
- 1.
A crosslinked streptolysin O obtained by crosslinking the streptolysin O consisting of:
- (a) a polypeptide consisting of an amino acid sequence selected from position 32 to position 464 of SEQ ID NO:
- 1, position 32 to position 466 of SEQ ID NO:
- 1, position 32 to position 471 of SEQ ID NO:
- 1, position 35 to position 464 of SEQ ID NO:
- 1, position 35 to position 466 of SEQ ID NO:
- 1, and position 35 to position 471 of SEQ ID NO:
- 1, or
- (b) a polypeptide having one or more tags in the N-terminal or C-terminal region of the amino acid sequence shown in (a).
A latex particle coated with the streptolysin O consisting of:
- (a) a polypeptide consisting of an amino acid sequence selected from position 32 to position 464 of SEQ ID NO:
- 1, position 32 to position 466 of SEQ ID NO:
- 1, position 32 to position 471 of SEQ ID NO:
- 1, position 35 to position 464 of SEQ ID NO:
- 1, position 35 to position 466 of SEQ ID NO:
- 1, and position 35 to position 471 of SEQ ID NO:
- 1, or
- (b) a polypeptide having one or more tags in the N-terminal or C-terminal region of the amino acid sequence shown in (a).
Dependencies are inferred from the claim text.