Synthesis of reversible nucleotide terminators by in-situ thio-alkyl group transfer for use in DNA sequencing by synthesis

Abstract

The invention is directed to a method for production of a deoxynucleotide triphosphate according to general formula (7) wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6 wherein R1, R2 and R3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B) wherein R4, R5 R6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BF4−, F−, Cl−, Br−, I−, SO42−, SO32− in aqueous solution having a pH between 3 and 7.

Dependencies are inferred from the claim text.