Synthetic DNA vectors and methods of use
AssigneeIntergalactic Therapeutics, Inc.
InventorsBruce C. Schnepp, Philip R. Johnson
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Abstract
Provided herein are isolated DNA vectors comprising a heterologous gene, wherein the DNA vector is devoid of bacterial plasmid DNA and/or bacterial signatures, which can abrogate persistence in vivo. The invention also features pharmaceutical compositions (non-immunogenic pharmaceutical compositions) including the DNA vectors of the invention, which can be used for induction of long-term, episomal expression of a heterologous gene in a subject. The invention involves methods of treating a subject by administering the DNA vectors of the invention, including methods of treating disorders associated with a defect in a target gene.
Claims28
A method of treating a cancer, the method comprising administering to a subject in need thereof a therapeutically effective amount of a synthetic circular DNA vector comprising two or more genes encoding two or more therapeutic polypeptides, wherein the synthetic circular DNA vector lacks an origin of replication, a drug resistance gene, and a site-specific recombination recognition site, thereby treating the cancer, wherein the two or more genes are together greater than 10 Kb in size.
Dependencies are inferred from the claim text.