Back to results
US 11,766,484 B2Granted
Exosome vessels for delivery of molecular cargo
AssigneeInternational Business Machines Corporation
InventorsNavneet Dogra, Gustavo Alejandro Stolovitzky
Life of this patent
Estimating the term…
Abstract
Techniques regarding the transportation and/or delivery of molecular cargo by exosomes are provided. For example, one or more embodiments described herein can comprise a molecule, which can comprise a chemically modified molecular cargo bonded to a surface biomolecule of an exosome. The surface biomolecule can be located on a bilayer membrane of the exosome opposite a cytoplasm of the exosome.
Claims9
A dual functionalized exosome, comprising:
- a synthetic nucleic acid bonded to a polypeptide chain of a first surface biomolecule of an exosome,
- wherein the first surface biomolecule is located at an external surface of a bilayer membrane of the exosome opposite a cytoplasm of the exosome,
- wherein the synthetic nucleic acid is bonded to a first functional group, the polypeptide chain of the first surface biomolecule is bonded to a second functional group, and the first functional group and the second functional group are coupled to one another to establish the bonding of the synthetic nucleic acid to the polypeptide chain of the first surface biomolecule, and
- wherein one of the first functional group or the second functional group comprises an antibody or an antigen, and the other of the first functional group or the second functional group comprises the other of the antibody or the antigen;
- and
- another antibody bonded to a polypeptide chain of a second surface biomolecule of the exosome, and
- wherein the other antibody has a chemical affinity to bond to a biological cell via an antibody-antigen interaction external to the bilayer membrane of the exosome.
4 more elements
A method, comprising:
- dually functionalizing an exosome, wherein the dual functionalization comprises
- bonding a synthetic nucleic acid to a first polypeptide chain of a first surface biomolecule,
- wherein the first surface biomolecule is located at an external surface of a bilayer membrane of the exosome opposite a cytoplasm of the exosome, and
- bonding an antibody to a second polypeptide chain of a second surface biomolecule of the exosome, and
- wherein the antibody has a chemical affinity to bond to a biological cell via an antibody-antigen interaction external to the bilayer membrane of the exosome.
2 more elements
A dual functionalized exosome, comprising:
- a synthetic nucleic acid bonded to a first polypeptide chain of a first surface biomolecule of an exosome,
- wherein the first surface biomolecule is located at an external surface of a bilayer membrane of the exosome opposite a cytoplasm of the exosome,
- wherein, to establish the bonding of the synthetic nucleic acid to the first polypeptide chain, the synthetic nucleic acid is bonded to a first functional group that is coupled to a second functional group of the first surface biomolecule, which second functional group is coupled to the first polypeptide chain,
- wherein the first functional group comprises a compound selected from a group consisting of an amine-reactive compound, a phosphate-reactive compound, a carbodiimide compound, and a hydroxyl-reactive compound, and
- wherein the second functional group comprises another compound selected from a second group consisting of a primary amine, a phosphate group, and a hydroxyl group;
- and
- an antibody bonded to a second polypeptide chain of a second surface biomolecule of the exosome, and
- wherein the antibody has a chemical affinity to bond to a biological cell via an antibody-antigen interaction external to the bilayer membrane of the exosome.
5 more elements
Dependencies are inferred from the claim text.