Q-peptide hydrogel promotes immune modulation and macrophage differentiation
AssigneeQuthero, Inc.
InventorsSerena Mandla, Milica Radisic
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Abstract
The present invention examines the interaction between an angiopoietin-1 mimetic peptide, QHREDGS (glutamine-histidine-arginine-glutamic acid-aspartic acid-glycine-serine (SEQ ID NO: 1)) immobilized to a collagen-chitosan hydrogel, and murine bone marrow derived macrophages. When macrophages were cultured in the presence of the peptide conjugated to a hydrogel, both pro-inflammatory and anti-inflammatory cytokines were produced, in contrast to the application of soluble peptide which elicited minimal cytokine secretion. This indicates a unique macrophage polarization with covalently immobilized peptide hydrogels, which can be beneficial in the context of the wound microenvironment.
Claims13
A method of inducing a polarization state of a macrophage, the method comprising contacting the macrophage with a formulation, wherein the formulation comprises a peptide that comprises the amino acid sequence QHREDGS (SEQ ID NO:
- 1) cross-linked to a carrier;
- wherein the carrier is selected from hydrogel, glycerol, propylene glycol, chitosan, alginate, agarose, polyether, polyester, methylcellulose, hyaluronan, collagen, laminin, fibronectin, vitronectin, poly-1-lysine, a proteoglycan, fibrin glue, a gel made by decellularization of an engineered or natural tissue, polyglycolic acid (PGA), polylactic acid (PLA), a combination of PGA and PLA, PLGA, poly ε-caprolactone, polyvinyl alcohol (PVA), polyethylene glycol (PEG), methyl methacrylate, poly(methyl methacrylate) (PMMA), poly(-hydroxyethyl methacrylate) (PolyHEMA), poly(glycerol sebacate), a self-assembling peptide hydrogel, AcN-RARADADARARADADA-CNH (SEQ ID NO:
- 2), polyurethane, poly(isopropylacrylamide), poly(N-isopropylacrylamide), and a combination thereof.
Dependencies are inferred from the claim text.