Method for gut mucosa preparation to enhance microbial engraftment

A method for microbiome transplantation and engraftment in an individual in need thereof, the method comprising:

• (a) washing out colonic fecal material from the colon, wherein said washing out comprises administering in an effective amount a formulation comprising a biofilm dissolving or disrupting agent to or into the colon, wherein the biofilm dissolving or disrupting agent comprises one or more agents selected from the group consisting of:
• soap in water, iodine, N-acetylcysteine, dispersin, ribonucleic-acid-III inhibiting peptide (RIP), Salvadora persica extracts, competence-stimulating peptide (CSP) patulin (PAT), penicillic acid (PA)/EDTA, cathelicidin-derived peptides, small lytic peptide (PTP)-/7, nitric oxide, cys-2-decenoic acid, sodium nitroprusside, s-nitroso-1-glutathione (GSNfaO), s-nitroso-N-acetylpenicillamine (SNAP), chlorhexidine, povidone-iodine (PI), a nanoemulsion, a lytic bacteriophage, a lactoferrin, a xylitol hydrogel, a synthetic iron chelator, a cranberry component, curcumin, acetyl-11-keto-boswellic acid (AKBA), a barley coffee (BC) component, silver nanoparticles, sinefungin, S-adenosylmethionine, S-adenosyl-homocysteine, a Delisea furanone, an N-sulfonyl homoserine lactone, iron salts, ionic silver salts, arsenicals, selenium, titanium dioxide, gallium nitrate, ethanol, hydrogen peroxide, hydrochloric acid, formaldehyde, luminal formalin, ozonated water, super-oxidized aqueous solution, nitrofurantoin, hexamine hippurate, potassium hydroxide, mercuric chloride, iodine, disodium EDTA, a combination of selenium and gentamicin, and a combination thereof;
• and
• (b) after washing out the colonic fecal material from the colon, administering to the individual in a therapeutically effective amount:
• (i) a fecal microbiota transplantation (FMT) material, liquid, formulation or solution, or
• (ii) a material, liquid, formulation or solution comprising a normal microbiome.