Ganglioside GM3-containing nanoparticles as immunomodulators

A pharmaceutical composition comprising nanoparticles, wherein each nanoparticle comprises outer membrane protein complex (OMPC) of Neisseria meningitidis bacterium hydrophobically conjugated to a GM3 ganglioside, wherein the protein-ganglioside conjugation ratio ranges from 1.5:

• 1 to 10:
• 1, and wherein the composition is characterized by a monomodal distribution of volume in a range from 15 to 25 nm particle size, polydispersity index of 0.230, negative Z potential with nominal value in the range from 25 to 45 mV.

A pharmaceutical composition comprising nanoparticles characterized by hydrophobic conjugation of outer membrane protein complex (OMPC) of Neisseria meningitidis bacterium to a GM3 ganglioside, wherein the nanoparticles are characterized by a monomodal distribution of volume in a range from 15 to 25 nm particle size, a polydispersity index of 0.230, and a negative Z potential with nominal value in the range from 25 to 45 mV.

A method of producing a nanopoarticle, the method comprising conjugating outer membrane protein complex (OMPC) of Neisseria meningitidis bacterium to a GM3 ganglioside, where the OMPC:GM3 ganglioside conjugation ratio ranges from 1.5:1 to 10:1.