Method for culturing natural killer cells using T cells
AssigneesGC Cell Corporation, Mogam Institute for Biomedical Research
InventorsBo Kyung Min, Hana Choi, Yu Kyeong Hwang
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Abstract
The present invention relates to a method for producing natural killer cells using T cells, and more particularly, to a method for producing natural killer cells, which comprises culturing seed cells using CD4(+) T cells as feeder cells. The method for producing natural killer cells using T cells according to the present invention is a method capable of producing natural killer cells by selectively proliferating only natural killer cells from a small amount of seed cells while maintaining the high killing activity of the natural killer cells. The method of the present invention can produce a large amount of natural killer cells that can be frozen, and thus is useful for commercialization of cell therapeutic agents.
Claims14
A method for treating cancer, the method comprising:
- administering to a subject in need thereof a therapeutically effective amount of a population of natural killer cells produced by the method comprising:
- (a) providing CD3(+)-depleted seed cells comprising mononuclear cells comprising natural killer cells;
- (b) expanding the natural killer cells by culturing the seed cells with a plurality of cells from an inactivated CD4(+) T cell line in a medium comprising:
- a T-cell stimulating antibody selected from the group consisting of OKT3, UCHT1, HTa, or a combination thereof;
- and
- IL-2,
- to produce expanded natural killer cells,
- thereby producing the population of natural killer cells.
Dependencies are inferred from the claim text.