Back to results
US 11,766,402 B2Granted
Methods for production of MSC-derived exosomes
AssigneeBoard of Regents, The University of Texas System
InventorsElizabeth Shpall, Raghu Kalluri, Katy Rezvani, Mayela Mendt, Valerie Lebleu, Sushrut Kamerkar
Life of this patent
Estimating the term…
Abstract
Provided herein are methods of manufacturing clinical grade exosomes derived from mesenchymal stem cells (MSCs). Further provided are methods of loading the exosomes with therapeutic agents, such as siRNA. Also provided herein are methods of treating diseases by administering the clinical grade exosomes.
Claims37
A method of manufacturing exosomes from mesenchymal stem cells (MSCs) comprising:
- (a) culturing the MSCs in a functionally closed bioreactor to 75-95% confluency in media comprising human platelet lysate (PLT);
- (b) further culturing the cells in media essentially free of PLT;
- (c) collecting one or more conditioned media fractions from the bioreactor wherein conditioned media fractions are collected every 24-48 hours;
- and
- (d) isolating exosomes from the conditioned media fractions.
2 more elements
Dependencies are inferred from the claim text.