Mitigating adverse effects of sunlight with ingredients obtained from living plants

A method of improving skin appearance associated with skin aging by mitigating the adverse effects of full spectrum sunlight exposure on skin cells, said method comprising the step of applying an anti-aging bioactive composition to a skin surface having at least one sign of aging,

• wherein said anti-aging bioactive composition comprises of i) a dermatologically acceptable carrier and ii) an effective amount of Chamomilla recutita (German Chamomile) serum fraction;
• wherein said Chamomilla recutita (German Chamomile) Flower serum fraction is isolated using a fractionation process which comprises subjecting plant cell juice derived from fresh plant biomass of the Chamomilla recutita (German Chamomile) flower to an electromagnetic field at a frequency of greater than 2.45 GHz for a time effective to destabilize the plant cell juice yielding a coagulated cell juice mixture comprising a coagulated membrane fraction, and separating said coagulated membrane fraction from said coagulated cell juice mixture in order to yield a bioactive fraction comprising a cytoplasm/cytosole fraction that is substantially-free from said membrane fraction, followed by additional treatments enabling to separate cytoplasm fraction from cytosole fraction, then stabilizing cytosole fraction to yield the Chamomilla recutita (German Chamomile) stabilized serum fraction;
• wherein said Chamomilla recutita stabilized serum fraction has a dry matter ranging from between 3.30-4.70% w/w, a total soluble sugar content ranging from between 1.33-1.89% w/w and a total phenolic compound content ranging from 0.10-0.15% w/w;
• wherein the said bioactive composition has a synergistic combination of properties selected from the group consisting of the following:
• (i) beneficial spectral absorbance characteristics in UVA-UVB area;
• (ii) high UVA:UVB absorbance ratios in conjunction with broad UVA and UVB spectral absorption photostability demonstrated after full spectrum simulated sun exposures;
• (iii) increased attenuation in UVA1 area and simultaneous increase in UVA/UVB ratio as radiation dose is increased;
• (iv) potent biological activities (properties) demonstrated in in vitro cell culture based ELISA (Enzyme-Linked Immuno Sorbent Assays) which measure the amount of cytokines (IL-6) and chemokines (IL-8) in response to full spectrum simulated sun exposure and relevant enzymatic models which measure the inhibition of elastase and MMP3 (Matrix Metalloproteinase-3);
• (v) maintenance of more than 95% of initial (pre-irradiation) DPPH (2,2-Diphenyl-1-Picrylhydrazyl) quenching capacities after 4 MED exposure delivered by full spectrum simulated sunlight;
• and (vi) multifunctional activities that work together to mitigate various adverse effects of full spectrum sunlight exposure on skin cells;
• and
• wherein said anti-aging bioactive composition is applied for a period of time sufficient to improve the appearance of the at least one sign of aging.