Reduced negative zeta potential sanitization method and system

A method for reducing the growth of bacteria and reversing the growth of biofilm in a water system, comprising:

• chlorinating source water and passing the chlorinated source water through a low zeta potential crystal generator and changing the crystalline structure of minerals in the source water to produce treated chlorinated nanobubble water having an enhanced concentration of low zeta potential crystal, and wherein the microbial content of the treated chlorinated nanobubble water is reduced by at least about 0.3 log CFU/mL compared to the source water;
• continuously circulating for a preselected period of time the treated chlorinated nanobubble water through a water system for processing food after the processing of food is complete;
• wherein the continuously circulating treated chlorinated nanobubble water has an initial free available chlorine level of at least 50 ppm;
• wherein, after the preselected period of time, the free available chlorine level is reduced to an operating level of 5 ppm;
• maintaining the available chlorine level at 5 ppm by infusing, as needed, the treated chlorinated nanobubble water with concentrated chlorine and continuously circulating the same through the water system;
• and
• bubbling carbon dioxide into the circulating treated chlorinated nanobubble water to maintain a target pH 5.0+/−0.5.