Modulated nutritional quality traits in seeds

A method of increasing crude fat content of a seed produced by a wheat plant, comprising mechanically or manually inoculating a wheat plant seed with a treatment formulation comprising an agriculturally compatible carrier and a purified bacterial population that comprises a seed bacterial endophyte of the genus Enterobacter that is heterologous to the wheat plant seed and comprises a polynucleotide sequence having at least 99% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO:

• 62, wherein the endophyte is heterologously disposed to the wheat plant seed in an amount effective to colonize a wheat plant germinated from the inoculated wheat plant seed, wherein a seed obtained from the colonized wheat plant exhibits an increase in crude fat content as compared to a reference wheat plant seed obtained from a reference wheat plant lacking the colonized endophyte of the genus Enterobacter, and wherein said reference wheat plant is germinated from a reference wheat plant seed lacking the colonized endophyte of the genus Enterobacter comprising the polynucleotide sequence having at least 99% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO:
• 62.