Mitigation of maize heat stress with recombinant 6-phosphogluconate dehydrogenase
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsAndrew Mark Settles, Camila Ribeiro
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Abstract
The disclosure relates to modification of a heat resistant cytoplasmic heat stable 6-phosphogluconate dehydrogenase (6PGDH) enzyme by fusing the cytoplasmic 6PGDH enzyme in frame to a plastid-targeting sequence. This modification allows the import of the cytoplasmic 6PGDH enzyme into plastids of a plant cell. Polynucleotides encoding and expressing the modified cytoplasmic 6PGDH enzymes are provided. The disclosure further provides transgenic plants and seeds containing the disclosed polynucleotides and expressing the modified cytoplasmic 6PGDH enzymes during development. The invention further relates to methods for developing a transgenic plant that has increased heat resistance and yield during heat stress.
Claims9
An expression cassette comprising a regulatory region operably linked to a nucleic acid sequence encoding a fusion protein comprising a plastid targeting sequence, wherein the plastid targeting sequence comprises a N-terminal chloroplast targeting sequence of starch synthase Waxy1 fused in frame with cytosolic 6-phosphogluconate dehydrogenase (6PGDH), wherein the regulatory region is a plant promoter.
A nucleic acid sequence encoding a fusion protein, wherein the fusion protein comprises a plastid targeting sequence, wherein the plastid targeting sequence comprises a N-terminal chloroplast targeting sequence of starch synthase Waxy1 fused in frame with cytosolic 6-phosphogluconate dehydrogenase (6PGDH), wherein the fusion protein is able to import into a plastid of a plant cell.
A transgenic plant comprising a nucleic acid sequence encoding a fusion protein, wherein the fusion protein comprises a plastid targeting sequence, wherein the plastid targeting sequence comprises a N-terminal chloroplast targeting sequence of starch synthase Waxy1jused in frame with cytosolic 6-phosphogluconate dehydrogenase (6PGDH), wherein the fusion protein is able to import into a plastid of a plant cell.
A method for developing a transgenic plant that has increased heat resistance and yield during heat stress, comprising introducing an expression cassette comprising a regulatory region operably linked to a nucleic acid sequence encoding a fusion protein comprising a plastid targeting sequence fused in frame with cytosolic 6-phosphogluconate dehydrogenase (6PGDH), wherein the plastid targeting sequence comprises N-terminal chloroplast targeting sequence of starch synthase Waxy1 or VVC.
Dependencies are inferred from the claim text.