Application of transgenic stem cell-derived exosome in preparing medicament or whitening cosmetic
AssigneeGuangdong Cell Biotechnology Co., Ltd.
InventorsXinyi Shi, Taihua Wang, Fei Peng, Jing Li
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Abstract
An application of a transgenic stem cell-derived exosome in preparing a medicament or whitening cosmetic is provided. In the present disclosure, miR-27b-3p is transfected into an epidermal stem cell, and a transgenic stem cell-derived exosome is harvested. It is experimentally verified that the exosome can inhibit the expression of PIK3R3 protein in melanocytes and the proliferation and migration of melanocytes; and safety experiments further demonstrate the safety of the exosome. Therefore, corresponding medicaments or cosmetics prepared from the exosome have excellent medicinal and cosmetic application prospects.
Claims2
A method of making a medicament comprising a miR-27b-3p-overexpressing epidermal stem cell-derived exosome, wherein the miR-27b-3p-overexpressing epidermal stem cell-derived exosome is prepared according to the following steps:
- step 1, transfecting miR-27b-3p into epidermal stem cells, and screening the epidermal stem cells for amiR-27b-3p-overexpressing epidermal stem cell;
- and
- step 2, culturing the miR-27b-3p-overexpressing epidermal stem cell obtained in step 1, collecting a supernatant, removing floating living cells by a centrifugation to obtain an exosome-containing supernatant, and centrifuging the exosome-containing supernatant to obtain the miR-27b-3p-overexpressing epidermal stem cell-derived exosome;
- a transfection is achieved by an electroporation or a liposome-mediated transformation;
- a melanocyte is an MU89 human melanocyte.
A method of making a cosmetic comprising a miR-27b-3p-overexpressing epidermal stem cell-derived exosome, wherein the miR-27b-3p-overexpressing epidermal stem cell-derived exosome is prepared according to the following steps:
- step 1, transfecting miR-27b-3p into epidermal stem cells, and screening the epidermal stem cells for amiR-27b-3p-overexpressing epidermal stem cell;
- and
- step 2, culturing the miR-27b-3p-overexpressing epidermal stem cell obtained in step 1, collecting a supernatant, removing floating living cells by a centrifugation to obtain an exosome-containing supernatant, and centrifuging the exosome-containing supernatant to obtain the miR-27b-3p-overexpressing epidermal stem cell-derived exosome;
- a transfection is achieved by an electroporation or a liposome-mediated transformation;
- a melanocyte is an MU89 human melanocyte.
Dependencies are inferred from the claim text.