Microfluidic chip and a method for the manufacture of a microfluidic chip

A microfluidic chip for performing a chemical or biochemical test in a metered reaction volume, the microfluidic chip comprising:

• a body defining an inner flow volume,
• an inlet provided to the body for connecting the inner flow volume to an ambient space, and
• a sample channel forming part of the inner flow volume and being connected to the inlet for the passage of fluid between the inlet and the sample channel,
• wherein,
• the microfluidic chip comprises a waste channel forming part of the inner flow volume and being connected to the inlet for the passage of fluid between the inlet and the waste channel,
• the sample channel comprises a first hydrophobic stop and a second hydrophobic stop at a distance from the first hydrophobic stop so as to provide a metered reaction volume there between,
• the microfluidic chip comprises an expelling channel connected to the metered reaction volume of the sample channel through the first hydrophobic stop for the passage of fluid between the expelling channel and the reaction volume,
• the microfluidic chip comprises a sample reservoir connected to the metered reaction volume of the sample channel through the second hydrophobic stop for the passage of fluid between the reaction volume and the sample reservoir, and
• the microfluidic chip comprises a pneumatic source in fluidic connection with the expelling channel, which pneumatic source is configured to introduce pressurized air into the inner flow volume so as to urge fluid in the metered reaction volume past the second hydrophobic stop.