Method and molecules
AssigneesMedImmune, LLC, The Regents of the University of California
InventorsRonald James Christie, Changshou Gao, Andre Henri St. Amant, Javier Read de Alaniz
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Abstract
The present invention provides a bioconjugation method and compounds for use therein. The bioconjugation method comprises the step of conjugating a biological molecule containing a first unsaturated functional group with a payload comprising a second unsaturated functional group, wherein the first and second unsaturated functional groups are complementary to each other such that conjugation is a reaction of said functional groups via a Diels-Alder reaction which forms a cyclohexene ring.
Claims5
An antibody or binding fragment thereof conjugated to a payload, wherein the conjugation reaction linking the antibody or binding fragment thereof to the payload was via a Diels-Alder reaction between a diene and a dieneophile to form a cyclohexene ring, and wherein the resulting conjugate is represented by formula (IVa):
- wherein
- RY represents the payload;
- and
- RZ represents the antibody or binding fragment thereof;
- or wherein the resulting conjugate is represented by formula (IVb):
- wherein
- RY represents the payload;
- RZ represents the antibody or binding fragment thereof, and
- X4 represents —CH2—.
Dependencies are inferred from the claim text.