Modified norovirus VP1 proteins and VLPs comprising modified norovirus VP1 proteins

A modified norovirus VP1 protein comprising an S domain and a P domain,

• wherein the S domain comprises an amino acid sequence of the S domain of a first wild type norovirus VP1 protein, wherein said sequence comprises one or more amino acid substitutions selected from the group consisting of substitutions at a position in sequence alignment with amino acids 39, 53 and 80 of a reference sequence of SEQ ID NO:
• 1;
• wherein the P domain comprises an amino acid sequence of the P domain of a second wild type norovirus VP1 protein, wherein said sequence comprises one or more amino acid substitutions selected from the group consisting of substitutions at a position in sequence alignment with amino acids 333 and 368 of the reference sequence of SEQ ID NO:
• 1;
• or
• a combination thereof, and
• wherein the amino acid substitution at the position in sequence alignment with amino acid 39 is to valine, isoleucine, leucine or methionine;
• the amino substitution at the position in sequence alignment with amino acid 53 is to isoleucine, leucine, valine, alanine or methionine;
• the amino substitution at the position in sequence alignment with amino acid 80 is to serine, asparagine, cysteine or threonine;
• the amino substitution at the position in sequence alignment with amino acid 333 is to valine, isoleucine or leucine;
• and the amino substitution at the position in sequence alignment with amino acid 368 is to glutamate, asparagine or aspartate.