Preparing and use of Glu-plasminogen from blood fractions

A method for isolating Glu-plasminogen, the method comprising the following steps:

• (i) providing a plasma fraction comprising Glu-plasminogen, wherein the plasma fraction is selected from the group consisting of:
• (a) cryo-poor plasma;
• and
• (b) fractions I+III of the Cohn or Kistler-Nitschmann process, fractions I+II+III of the Cohn or Kistler-Nitschmann process, or a combination thereof, wherein, optionally, octanoic acid is added to the fractions I+III or the fractions I+II+III;
• (ii) contacting the plasma fraction with an anion exchanger based on a resin comprising cationic groups;
• (iii) washing the anion exchanger obtained from step (ii) loaded with the plasma fraction with a first buffer B1 having a pH of 8.5 to 11 not comprising cations competing with the cationic groups of the resin of the anion exchanger;
• (iv) eluting the Glu-plasminogen from the washed anion exchanger of step (iii) with a second buffer B2 having a pH of 8.5 to 11 comprising cations competing with the cationic groups of the resin of the anion exchanger, thereby obtaining a solution comprising the second buffer B2 and Glu-plasminogen;
• (v) adjusting the pH of the solution obtained from step (iv) to a pH in a desired range of pH 4.5 to 6.5;
• (vi) stabilizing the Glu-plasminogen by adding one or more stabilizers that prevent the Glu-plasminogen from maturing into plasmin and Lys-plasminogen to the solution obtained from step (iv) and/or step (v);
• (vii) optionally subjecting the solution from any of steps (iv) to (vi) to one or more antiviral treatments;
• and
• (viii) optionally drying or freeze-drying the solutions comprising Glu-plasminogen obtained from any of steps (iv) or (vii).