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US 11,754,570 B2Granted
Methods for quantitation of functional C1 esterase inhibitor (FC1-INH)
AssigneeTakeda Pharmaceutical Company Limited
InventorsPriya Sethu Chockalingam, Yongquan Lai, Jiang Wu, Guodong Zhang, Zhiwei Zhou
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Abstract
Methods for quantitation of fC1-INH from dried blood spot are provided herein. Such methods may comprise spotting and drying a blood sample on a support member, extracting protein from the dried blood sample and measuring the level of fC1-INH in the extracted proteins.
Claims20
A method for determining a level of functional C1-esterase inhibitor (fC1-INH) in a sample, the method comprising:
- (i) spotting a blood sample from a subject on a support member;
- (ii) drying the blood sample on the support member to form a dried blood spot;
- (iii) extracting proteins from the dried blood spot from (ii);
- and
- (iv) measuring the level of fC1-INH in the extracted proteins in (iii), if present;
- wherein measuring the level of fC1-INH comprises:
- (a) incubating the extracted proteins with a complement component 1s (C1s) and a C1s substrate, to produce a C1s substrate product;
- (b) measuring the level of the C1s substrate product produced in step (a);
- and
- (c) determining the level of fC1-INH in the dried blood spot based on the level of the C1s substrate product measured in step (b).
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Dependencies are inferred from the claim text.