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US 11,754,499 B2Granted
Enzyme quantification
AssigneeBio-Rad Laboratories, Inc.
InventorsDarren Roy Link, Michael L. Samuels
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Abstract
The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.
Claims12
A method for identifying components of a chemical reaction, the method comprising:
- forming fluid partitions comprising components of a chemical reaction, wherein said components comprise:
- an enzyme that catalyzes said chemical reaction, and a detectable label that is acted on by said chemical reaction;
- conducting said chemical reaction;
- and
- determining an amount of said enzyme in said fluid partitions based upon one or more properties of the detectable label in said fluid partitions.
2 more elements
Dependencies are inferred from the claim text.