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US 11,753,659 B2Granted
Glycerol and acetic acid converting yeast cells with improved acetic acid conversion
AssigneeDsm IP Assets B.V.
InventorsPaul Klaassen, Wouter Willem Antonius Hartman
Life of this patent
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Abstract
Cell that is genetically modified comprising: a) one or more nucleotide sequence encoding a NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); b) one or more nucleotide sequence encoding a acetyl-CoA synthetase (E.C. 6.2.1.1); c) one or more nucleotide sequence encoding a glycerol dehydrogenase (E.C. 1.1.1.6); and d) one or more nucleotide sequence encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 or E.C. 2.7.1.29).
Claims13
A yeast cell that is genetically modified comprising:
- a) one or more nucleotide sequence encoding a heterologous ethanolamine utilization protein having the amino acid sequence set forth in SEQ ID NO:
- 3 or a functional homologue thereof having at least 80% amino acid identity to SEQ ID NO:
- 3;
- b) one or more nucleotide sequence encoding a acetyl-CoA synthetase (belonging to E.C. 6.2.1.1);
- c) one or more nucleotide sequence encoding a glycerol dehydrogenase (belonging to E.C. 1.1.1.6);
- and
- d) one or more nucleotide sequence encoding a dihydroxyacetone kinase (belonging to E.C. 2.7.1.28 or E.C. 2.7.1.29), wherein the one or more recombinant nucleotide sequence encoding the dihydroxyacetone kinase is overexpressed in the yeast cell, and
- wherein the yeast cell consumes glucose, xylose, glycerol and acetic acid.
5 more elements
Dependencies are inferred from the claim text.