Host cell capable of producing enzymes useful for degradation of lignocellulosic material

A process for preparation of a transformed filamentous fungus fungal host cell comprising:

• (a) Providing one or more expression cassettes comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases and the control sequences required for expression of these polynucleotides;
• (b) Providing a selectable marker;
• (c) Transforming filamentous fungal host cells with the one or more expression cassettes and the selectable marker from (b);
• and
• (d) Selecting at least one transformed filamentous fungal host cell that produces the at least four different cellulases, hemicellulases and/or pectinases encoded by the at least four different heterologous polynucleotides,
• wherein the transformed filamentous fungal host cell from (d) is capable of secreting the at least four different fungal enzymes, and the filamentous fungal host cells transformed are an Aspergillus strain or a Trichoderma strain.