Medium supplement to increase the efficiency of oocyte maturation and embryo culture in vitro
AssigneeThe Curators of the University of Missouri
InventorsYe Yuan, Lee D. Spate, Randall S. Prather, R. Michael Roberts
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Abstract
The present invention provides a novel oocyte maturation medium or/and embryo culture medium with a chemically defined supplement to produce matured oocytes at high efficiency. The inventive medium or supplement comprises three growth factors, namely, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF-1) in a synergistic combination. Methods for oocyte and embryo culture are also provided.
Claims22
A medium for culturing an oocyte comprising fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF-1), in an amount effective to provide increased maturation of an oocyte therein compared to a control medium.
A medium for culturing an embryo comprising fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF-1), in an amount effective to provide increased zygote development to a blastocyst compared to a control medium.
Dependencies are inferred from the claim text.