Alkali-tolerant affinity chromatography ligands
AssigneeBioprocessia Technologies LLC
InventorYinong Zong
Life of this patent
Estimating the term…
Abstract
The Sequence Listing associated with this application is provided in text form in lieu of a paper copy, and is hereby incorporated by reference into the specification. The name of the text file containing the Sequence Listing is SequenceListing.txt. The text file is 36.3 KB, and was created on Sep. 30, 2020.
Claims19
A polypeptide comprising a first immunoglobulin (Ig)-binding domain, wherein the first Ig-binding domain comprises:
- (1) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1, wherein the amino acid that corresponds to S44 of SEQ ID NO:
- 1 is hydrophobic;
- (2) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2, wherein the amino acid that corresponds to S43 of SEQ ID NO:
- 2 is hydrophobic;
- (3) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 3, wherein the amino acid that corresponds to S41 of SEQ ID NO:
- 3 is hydrophobic;
- (4) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 4, wherein the amino acid that corresponds to S41 of SEQ ID NO:
- 4 is hydrophobic;
- (5) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 5, wherein the amino acid that corresponds to S41 of SEQ ID NO:
- 5 is hydrophobic;
- or
- (6) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 6, wherein the amino acid that corresponds to S41 of SEQ ID NO:
- 6 is hydrophobic.
A polypeptide comprising an immunoglobulin (Ig)-binding domain, wherein the Ig-binding domain comprises:
- (1) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1, wherein the amino acid that corresponds to N55 of SEQ ID NO:
- 1 is Val;
- (2) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2, wherein the amino acid that corresponds to N54 of SEQ ID NO:
- 2 is Val;
- (3) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 3, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 3 is Val;
- (4) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 4, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 4 is Val;
- (5) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 5, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 5 is Val;
- or
- (6) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 6, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 6 is Val,
- wherein the amino acid that corresponds to S44 of SEQ ID NO:
- 1, S43 of SEQ ID NO:
- 2, S41 of SEQ ID NO:
- 3, S41 of SEQ ID NO:
- 4, S41 of SEQ ID NO:
- 5 or S41 of SEQ ID NO:
- 6 is hydrophobic.
A polypeptide comprising an immunoglobulin (Ig)-binding domain, wherein the Ig-binding domain comprises SEQ ID NO:
- 6 with a mutation at S41, wherein S41 is substituted with a hydrophobic amino acid residue.
Dependencies are inferred from the claim text.