Back to results
US 11,747,352 B2Granted
Eplerenone for phenotyping of CYP3A5 enzyme activity in saliva samples
AssigneeConcordia University
InventorsJoseph McGraw, Armin Gerhardt
Life of this patent
Estimating the term…
Abstract
Disclosed are methods and compositions which may be used in human cytochrome P450 (CYP450) enzyme phenotyping. The methods and compositions typically utilize substrate for CYP3A5 comprising eplerenone which may be administered orally to a subject. Subsequently, metabolites of eplereone may be detected in the subject's saliva as well as any non- metabolized eplerenone to calculate a metabolic ratio for CYP3A5 enzyme in order to generate a phenytopic CYP3A5 enzyme profile for the subject.
Claims8
A method comprising:
- (a) administering orally to a subject a composition comprising eplerenone;
- (b) three to four hours after administration, collecting a saliva sample from the subject;
- (c) subjecting the saliva sample to one or more procedures selected from Ultra High Pressure Liquid Chromatography (UHPLC), Mass Spectroscopy (MS), High Pressure Liquid Chromatography (HPLC), Ultraviolet Spectroscopy (UV), Gas Chromatography (GC), Electron Capture Detection (ECD), Flame Ionization Detection (FID), Raman Infrared (RI) Spectroscopy, Matrix-Assisted Laser Desorption/Ionization (MALDI), immunoassay analytical techniques, wherein the procedure provides a saliva concentration of (i) non-metabolized eplerenone;
- (ii) 6β-hydroxyeplerenone;
- and (iii) 21-hydroxyeplerenone.
2 more elements
Dependencies are inferred from the claim text.