Microarray based sample detection system

A microarray assembly for detection of a target molecule in a sample, comprising:

• an amplification chamber with a sample inlet, a sample outlet, a top interior surface, a bottom interior surface, side walls and a microarray located on the bottom interior surface, wherein the microarray is a gel spot microarray, wherein nucleic acids are amplified in the amplification chamber and hybridize to the microarray and wherein the top interior surface is a hydrophilic surface that facilitates complete filling of the amplification chamber by an aqueous fluid;
• a one-way valve at the sample inlet to prevent environmental contamination;
• a waste chamber comprising a waste inlet and an absorbent material;
• and
• a channel connecting the amplification chamber to the waste chamber, the channel having an expansion section with a first end proximate to the outlet of the amplification chamber and a second end proximate to the inlet of the waste chamber,
• wherein the top interior surface of the amplification chamber is a hydrophilic surface that facilitates complete filling of the amplification chamber by an aqueous fluid and wherein the cross-sectional area at the first end of the expansion section is smaller than the cross-sectional area at the second end of the expansion section.