Methods and systems for analyte detection and analysis

A system for analyte detection or analysis, comprising:

• a chamber configured to receive a substrate comprising a plurality of analytes immobilized thereon;
• a rotational unit configured to rotate the substrate about a rotational axis;
• and
• a detector comprising one or more sensors configured to image the substrate during rotation of the substrate to detect a plurality of signals from the plurality of analytes, wherein the one or more sensors comprise a plurality of segments, wherein, during imaging,
• (i) an imaging field of view comprises at least a first region and a second region which are disposed at different radial distances with respect to the rotational axis, and
• (ii) the detector is configured to use a first segment and a second segment of the plurality of segments to image the first region and the second region, respectively, by independently clocking the first segment and the second segment with respect to each other based at least in part on different tangential velocities of the first region and the second region, respectively, at the different radial distances.