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US 11,746,344 B2Granted
Method for producing isoprene using transformed E. coli
AssigneeIndustry-Academic Cooperation Foundation Gyeongsang National University
InventorsSeon Won Kim, Jung Hun Kim, Myeong Seok Cha, Hui Jeong Jang
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Abstract
A method for producing isoprene includes culturing E. coli, which has isoprene productivity and in which a gene encoding a recA protein is attenuated or deleted, in a medium containing a carbon source. Therefore, a great amount of isoprene may be produced within a short period of time, and thereby considerably decreasing isoprene production unit costs.
Claims31
An E. coli transformant comprising:
- foreign genes which are introduced into the E. coli to make the E. coli capable of producing the isoprene, the foreign genes comprising:
- a first foreign gene encoding isoprene synthase;
- a second foreign gene encoding an enzyme with function of acetyacetyl-CoA synthase;
- a third foreign gene encoding an enzyme with function of HMG-CoA reductase, wherein the second foreign gene and the third foreign gene are optionally a single gene encoding an enzyme with functions of both the acetyacetyl-CoA synthase and the HMG-CoA reductase;
- a fourth foreign gene encoding HMG-CoA synthase;
- a firth foreign gene encoding mevalonate kinase;
- a sixth foreign gene encoding mevalonate diphosphate carboxylase;
- a seventh foreign gene encoding phosphomevalonate kinase;
- and
- an eighth foreign gene encoding isoprenyl pyrophosphate isomerase;
- and
- wherein, in the E. coli transformant, a gene encoding a recA protein is attenuated or deleted in the E. coli to reduce or remove expression of the gene encoding the recA protein;
- and
- the E. coli transformant produces increased amounts of the isoprene, compared to wild type E. coli.
11 more elements
An E. coli transformant comprising a deletion of the gene encoding a recA protein and comprising the nucleotide sequence of SEQ ID NO:
- 1 encoding isoprene synthase, the nucleotide sequence of SEQ ID NO:
- 2 encoding an enzyme with functions of acetoacetyl-CoA synthase and HMG-CoA reductase, the nucleotide sequence of SEQ ID NO:
- 3 encoding HMG-CoA synthase, the nucleotide sequence of SEQ ID NO:
- 4 encoding mevalonate kinase, the nucleotide sequence of SEQ ID NO:
- 5 encoding mevalonate diphosphate carboxylase, the nucleotide sequence of SEQ ID NO:
- 6 encoding phosphomevalonate kinase, and the nucleotide sequence of SEQ ID NO:
- 7 encoding isoprenyl pyrophosphate isomerase,
- where the E. coli transformant produces increased amounts of isoprene compared to wild type E. coli.
5 more elements
Dependencies are inferred from the claim text.