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US 11,746,342 B2Granted
Method for manufacturing protein
AssigneeAjinomoto Co., Inc.
InventorsHiroaki Fukada, Hiroka Koguchi, Mitsunori Tokura
Life of this patent
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Abstract
A method for producing a protein is provided. An objective protein is produced by culturing Talaromyces cellulolyticus having an objective protein-producing ability, which has been modified so that the activity of a YscB protein is reduced, in a culture medium.
Claims15
A method for producing an objective protein, comprising
- culturing Talaromyces cellulolyticus having an ability to produce an objective protein in a culture medium,
- wherein the Talaromyces cellulolyticus has been modified so that the protease activity of a YscB protein is reduced as compared with a non-modified Talaromyces cellulolyticus;
- wherein the YscB protein is selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 43;
- (b) a protein comprising the amino acid sequence of SEQ ID NO:
- 43, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein has a protease activity;
- and
- (c) a protein comprising an amino acid sequence having an identity of 95% or higher to the amino acid sequence of SEQ ID NO:
- 43, and wherein said protein has a protease activity.
7 more elements
Dependencies are inferred from the claim text.