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US 11,746,336 B2Granted
Producing 3-hydroxycarboxylic acid and ketone using polyketide synthases
AssigneeThe Regents of the University of California
InventorsSatoshi Yuzawa, Leonard Katz, Jay D. Keasling
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Abstract
The present invention provides for a polyketide synthase (PKS) capable of synthesizing a 3-hydroxycarboxylic acid or ketone. The present invention also provides for a host cell comprising the PKS and when cultured produces the 3-hydroxycarboxylic acid or ketone.
Claims13
A non-naturally occurring polyketide synthase (PKS) capable of synthesizing a 3-hydroxycarboxylic acid or ketone;
- wherein the PKS comprises a loading acyltransferase (ATL) domain and a loading ACP (ACPL) domain, both of LipPk1 of a lipomycin synthase of Streptomyces aureofaciens T0117, a ketosynthase (KS) domain, an acyltransferase (AT) domain, a ketoreductase (KR) domain, an ACP domain of LipPks1 of a lipomycin synthase of Streptomyces aureofaciens Tü117, and a thioesterase (TE) domain appended to the C-terminus of the ACP domain of LipPks1;
- wherein (a) the AT domain is an AT domain from module 1 of borrelidin PKS, (b) the KR domain is a mutated KR domain being a KR domain of LipPks1 of a lipomycin synthase of Streptomyces aureofaciens Tü117 having an active-site scrims residue mutated to alanine such that the amino add sequence of SEQ ID NO:
- 7 comprised in the KR domain of LipPks1 is mutated to SEQ ID NO:
- 9 in the mutated KR domain, or both (a) and (b).
1 more element
A replicon comprising the recombinant nucleic acid 7, wherein the replicon is capable of stable maintenance in a host cell.
Dependencies are inferred from the claim text.