Method of forming high-throughput 3D printed microelectrode array
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsSwaminathan Rajaraman, Avra Kundu, Adam Rozman, Jorge Manrique Castro
Life of this patent
Estimating the term…
Abstract
A method of forming a high-throughput, three-dimensional (3D) microelectrode array for in vitro electrophysiological applications includes 3D printing a well plate having a top face and bottom face. A plurality of culture well each includes a plurality of 3D printed, vertical microchannels and microtroughs communicating with the microchannels. The microtroughs and the microchannels are filled with a conductive paste to form self-isolated microelectrodes in each of the culture wells and conductive traces that communicate with the self-isolated microelectrodes.
Claims24
A method of forming a high-throughput, three-dimensional (3D) microelectrode array for in vitro electrophysiological applications, comprising:
- 3D printing a well plate having a top face, a bottom face and a plurality of culture wells on the top face, and each culture well including a plurality of 3D printed, vertical microchannels on the top face, and microtroughs on the bottom face communicating with the microchannels, wherein the well plate and the plurality of culture wells are 3D printed onto a support structure that is positioned such that 3D printing occurs from the culture wells upward to the well plate using a bottom-up 3D printing process at a 0 to 12 degree orientation tilt angle relative to the well plate as a base plane;
- and
- filling the microtroughs and the microchannels with a conductive paste to form self-isolated microelectrodes in each culture well and conductive traces that communicate with the self-isolated microelectrodes.
A method of forming a high-throughput, three-dimensional (3D) microelectrode array for in vitro electrophysiological applications, comprising:
- 3D printing a well plate having a top face and a bottom face;
- forming on the top face a plurality of culture wells, wherein each culture well includes a plurality of 3D printed, vertical microchannels on the top face, and microtroughs on the bottom face communicating with the microchannels, wherein the well plate and the plurality of culture wells are 3D printed onto a support structure that is positioned such that 3D printing occurs from the culture wells upward to the well plate using a bottom-up 3D printing process at a 0 to 12 degree orientation tilt angle relative to the well plate as a base plane;
- ultraviolet curing the 3D printed well plate, the culture wells, the microchannels and the microtroughs;
- and filling the microtroughs and the microchannels with a conductive paste to form self-isolated microelectrodes in each culture well and conductive traces that communicate with the self-isolated microelectrodes.
A method of forming a high-throughput, three-dimensional (3D) microelectrode array for in vitro electrophysiological applications, comprising:
- forming by 3D printing a well plate having a top face, a bottom face, and a plurality of culture wells on the top face;
- and each culture well including a plurality of 3D printed, vertical microchannels on the top face, and microtroughs on the bottom face communicating with the microchannels, wherein the well plate and the plurality of culture wells are 3D printed onto a support structure that is positioned such that 3D printing occurs from the culture wells upward to the well plate using a bottom-up 3D printing process at a 0 to 12 degree orientation tilt angle relative to the well plate as a base plane;
- ultraviolet curing the 3D printed well plate, the culture wells, the microchannels and the microtroughs;
- and filling the microtroughs and the microchannels with a conductive paste to form self-isolated microelectrodes in each culture well and conductive traces that communicate with the self-isolated microelectrodes, each of said self-isolated microelectrodes having an electroplated top surface.
Dependencies are inferred from the claim text.