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US 11,744,887 B2Granted
Coronavirus vaccine compositions and methods
AssigneeArcturus Therapeutics, Inc.
InventorsSean Michael Sullivan, Daiki Matsuda, Kiyoshi Tachikawa, Padmanabh Chivukula, Priya Prakash Karmali, Jared Henry Davis, Yanjie Bao, Amit Sagi
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Abstract
Provided herein are nucleic acid molecules encoding viral replication proteins and antigenic coronavirus proteins or fragments thereof. Also provided herein are compositions that include nucleic acid molecules encoding viral replication and antigenic proteins, and lipids. Nucleic acid molecules provided herein are useful for inducing immune responses.
Claims28
A nucleic acid molecule comprising
- (a) a sequence of SEQ ID NO:124;
- (b) a sequence of SEQ ID NO:124, wherein T is substituted with U;
- (c) a sequence of SEQ ID NO:125;
- or
- (d) a sequence of SEQ ID NO:125, wherein T is substituted with U.
2 more elements
A composition comprising:
- (I) a nucleic acid molecule comprising:
- (A) a first polynucleotide encoding one or more viral replication proteins, wherein the first polynucleotide is codon-optimized as compared to a wild-type polynucleotide encoding the one or more viral replication proteins;
- and
- (B) a second polynucleotide comprising a transgene encoding an antigenic protein or a fragment thereof, wherein the antigenic protein is a coronavirus protein;
- and
- (II) a lipid formulation selected from a lipoplex, a liposome, a lipid nanoparticle, a polymer-based carrier, an exosome, a lamellar body, a micelle, and an emulsion,
- wherein the nucleic acid molecule comprises
- (a) a sequence of SEQ ID NO:124;
- (b) a sequence of SEQ ID NO:124, wherein T is substituted with U;
- (c) a sequence of SEQ ID NO:125;
- or
- (d) a sequence of SEQ ID NO:125, wherein T is substituted with U.
9 more elements
A lipid nanoparticle composition comprising
- (a) a lipid formulation comprising
- (i) about 45 mol % to about 55 mol % of an ionizable cationic lipid having the structure of ATX-126:
- (ii) about 8 mol % to about 12 mol % DSPC;
- (iii) about 35 mol % to about 42 mol % cholesterol;
- and
- (iv) about 1.25 mol % to about 1.75 mol % PEG2000-DMG;
- and
- (b) a nucleic acid molecule having at least 85% sequence identity to SEQ ID NO:125;
- wherein the lipid formulation encapsulates the nucleic acid molecule and the lipid nanoparticle has a size of about 60 to about 90 nm.
6 more elements
Dependencies are inferred from the claim text.